Both technologies yield long reads, the median and highest read lengths being 10 kilobases and 60 kilobases respectively for PacBio sequencing54. For nanopore the median read lengths of 10 to 12 kilobases55,56 are similar to PacBio, but in it can also yield ultra-long reads of 1 up to 2.3 megabases long57–59. The length of the reads and parallel nature of these two technologies allow these sequencers to have truly massive throughputs. PacBio sequencers can sequence between 2 and 11 gigabases per hour and ONT from 12.5 gigabases per hour, up to a staggering 260 gigabases per hour using the latest ONT PromethION machines56. The cost of sequencing with these machines, while higher than for Illumina sequencers, remains reasonably affordable at $0.32 and $0.13 per megabase for PacBio and ONT respectively60. These characteristics are summarized in Table 1.1 along with other sequencing technologies.
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